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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy - a 2 minute Preview of the Experimental Protocol
Frank W. S. Stetter, Sandra Kienle, Stefanie Krysiak, Thorsten Hugel
Technische Universität München, Physik-Department E22a; Technische Universität München, IMETUM;
A protocol to couple a large variety of single molecules covalently onto an AFM tip is presented. Procedures and examples to determine the adhesion force and free energy of these molecules on solid supports and bio-interfaces are provided.
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