High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography) is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material.
The Components of HPLC
1)Columns
2) Sample Injector
3) Reservoir
4) Pump
5) Detector
6) Data collection systems
Types of HPLC
Normal phase
Reverse phase

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