An introduction to RNA-seq analysis using in-house software tools.
RNA-Seq is a next-generation sequencing (NGS) technique designed to quantify RNA in a biological sample. As with any NGS technique, it requires appropriate data processing and statistical analysis. In this two-part training series, we provide a brief overview of a typical RNA-seq experiment and present two LMB-designed software tools: PRAGUI and PEAT.
The PRAGUI pipeline takes raw sequencing data, trims it, aligns it to the genome and quantifies expression and differential expression. PEAT then loads this data to allow for easy and user-friendly analysis of the results.
This first video is the webinar presented on 20/08/2020. Here, we explain the features of both software tools and provide basic instructions on how to use them.
The second video is a PRAGUI practical that will take you through the process of running PRAGUI step-by-step with some test data. You can find this here:
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You can find more webinars from scientists at the LMB here:
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About the MRC Laboratory of Molecular Biology (LMB):
The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Its scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health.
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