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Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration - a 2 minute Preview of the Experimental Protocol

Anna Holubowska, Chaitali Mukherjee, Mayur Vadhvani, Judith Stegmüller
Max Planck Institute of Experimental Medicine, Cellular and Molelcular Neurobiology; Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB),;

Neuronal morphogenesis and migration are crucial events underlying proper brain development. Here, we describe methods to genetically manipulate cultured cerebellar granule neurons and the developing cerebellum for the assessment of morphology and migratory characteristics of neurons.

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