Quadrant Streaking is performed microbiology where an inoculum is streaked over the agar surface in such a way that it “thins out” the bacteria. Some individual bacterial cells are separated and well-spaced from each other, which are referred to a "Single Colonies" which have arisen from one single bacterium. As the original sample is diluted by streaking it over successive quadrants, the number of organisms decreases. Usually, by the third or fourth quadrant, only a few organisms are transferred which will give discrete colony forming units (CFUs).
