A single-cell resolution dorsal view of convergent extension in a developing zebrafish embryo from 8.5 to 18.5 hours post fertilization
Convergent extension is the key process of making body plans from 3 germ layers. This movie provides a single-cell resolution dorsal view of this process in a developing zebrafish embryo from 8.5 to 18.5 hours post fertilization (hpf). Movie is a continuous sequence of 300 images with time stamp (unit:hours and minutes post fertilization, YSL, yolk syncytial layer). Each image is a 3D projection of a Z-stack (71μm deep) of 2-photon slices (400μm in height and width, Scale bar: 20μm) at 2 minute time intervals. Cell membranes were labelled with membrane targeted yellow fluorescent protein (coloured cyan in the movie) and cell nucleus with histone2B tagged red fluorescent protein (coloured yellow in the movie). These markers are excited by 2 photon laser at 1020nm. This movie allows researchers to directly follow the cell divisions and movements, changes of cell shapes, as well as the formation of different tissues. Movie courtesy Fengzhu Xiong and Sean Megason (Harvard Medical School).
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