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A study was conducted to assess the performance of various mixing regimes on methanogen biomass content in anaerobic digesters. Methane production in anaerobic digesters is directly related to the methanogens within the system. Current systems involve mixing to increase biogas production and system efficiency, however little is known about the underlying mechanisms of this relationship. In this study three pilot scale anaerobic digestion systems with three different mixing regimes were run with replication to examine the impacts to methanogen biomass content and biogas production. The results will provide insight for operational recommendations as well as the basic microbial processes with digestion systems which are critical for optimization.

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