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A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence - a 2 minute Preview of the Experimental Protocol

Zachary J. Storms, Elliot Cameron, Hector de la Hoz Siegler, William C. McCaffrey
University of Alberta, Department of Chemical and Materials Engineering; University of Calgary, Department of Chemical and Petroleum Engineering;

A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.

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