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Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA - a 2 minute Preview of the Experimental Protocol

Marc Herb, Alina Farid, Alexander Gluschko, Martin Krönke, Michael Schramm
University of Cologne, Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne; Center for Molecular Medicine Cologne,; Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD),; German Center for Infection Research (DZIF),;

Macrophages, especially primary macrophages, are challenging to transfect as they specialize in detecting molecules of non-self origin. We describe a protocol that allows highly efficient transfection of primary macrophages with mRNA generated from DNA templates such as plasmids.

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