University-level explanation of how DNA replication begins, talking about why each enzyme is important, both in prokaryotes (focusing on dnaA, dnaB (helicases) and dnaC), and in eukaryotes (explaining ORC1-6, mcm2-7 (helicases), cdc6 and cdt1, cdc45 and GINS, cdk, the pre-replication complex (pre-RC) and the helicase CMG complex), and how everything is regulated in the G1 and S phases so that each section of the DNA is only replicated once.
Spanish versions:
Prokaryotes: [ Ссылка ]
Eukaryotes: [ Ссылка ]
Website: [ Ссылка ]
0:00 - Introduction
1:10 - Prokaryotic origins
2:21 - dnaA proteins
3:38 - How dnaA proteins separate DNA strands
5:27 - dnaB (Prokaryotic helicase) and dnaC
7:05 - Eukaryotic origins
8:52 - ORC1-6
10:17 - mcm2-7 (Eukaryotic helicase)
10:55 - Cdc6, Cdt1 and Pre-RC
11:19 - S phase and CDKs
13:25 - Cdc45, GINS and CMG helicase complex
14:25 - Competency and efficiency of origins
15:20 - Geminin (GMNN)
16:07 - Farewell
