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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies - a 2 minute Preview of the Experimental Protocol
Belle M. Sharon, Neha V. Hulyalkar, Vivian H. Nguyen, Philippe E. Zimmern, Kelli L. Palmer, Nicole J. De Nisco
University of Texas at Dallas, Department of Biological Sciences; University of Texas Southwestern Medical Center, Department of Urology;
This protocol details a comprehensive approach for the culturing, sequencing, and de novo hybrid genome assembly of urinary bacteria. It provides a reproducible procedure for the generation of complete, circular genome sequences useful in studying both chromosomal and extrachromosomal genetic elements contributing to urinary colonization, pathogenesis, and antimicrobial resistance dissemination.
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