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Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum - a 2 minute Preview of the Experimental Protocol
Xuehua Xu, Tian Jin
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Chemotaxis Signal Section, Laboratory of Immunogenetics;
Here, we describe detailed live cell imaging methods for investigating chemotaxis. We present fluorescence microscopic methods to monitor spatiotemporal dynamics of signaling events in migrating cells. Measurement of signaling events permits us to further understand how a GPCR-signaling network achieves gradient sensing of chemoattractants and controls directional migration of eukaryotic cells.
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