Video demonstrating all steps required for the intravenous injection of hard-shelled amniote eggs.
This video corresponds to the Supplemental Video S1 in the article ‘Protocol for the rapid intravenous in-ovo injection of developing amniote embryos’, Cooper, Santos Durán & Milinkovitch — STAR Protocols 2023.
This technique was used to induce scale-to-feather transition in the embryonic chicken; the corresponding article is: ‘Transient agonism of the sonic hedgehog pathway triggers a permanent transition of skin appendage fate in the chicken embryo’, Cooper & Milinkovitch — Science Advances 2023 (DOI: 10.1126/sciadv.adg9619).
