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Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering - a 2 minute Preview of the Experimental Protocol
Tracy A. Gwyther, Jason Z. Hu, Kristen L. Billiar, Marsha W. Rolle
Worcester Polytechnic Institute, Biomedical Engineering Department;
This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.
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