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Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy - a 2 minute Preview of the Experimental Protocol
Ayse Sena Mutlu, Tao Chen, Dinghuan Deng, Meng C. Wang
Baylor College of Medicine, Huffington Center on Aging; Baylor College of Medicine, Department of Molecular and Human Genetics; Baylor College of Medicine, Howard Hughes Medical Institute;
Stimulated Raman scattering (SRS) microscopy allows selective, label-free imaging of specific chemical moieties and it has been effectively employed to image lipid molecules in vivo. Here, we provide a brief introduction to the principle of SRS microscopy and describe methods for its use in imaging lipid storage in Caenorhabditis elegans.
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