Watch how human neutrophils seeded in SeedEZ chemotax in 3D in a gradient of Interleukin 8. Neutrophils are the most abundant type of white blood cells in the circulation. They are an essential component of the innate immune system protecting the host against infection and responding early to inflammation.
Lena Biosciences platform technologies allow to screen agents modulating directional decision of cells to respond to soluble factors by attraction and/or repulsion and to study when and why cells make these decisions, or alter them in real time in response to chemokines, drugs, and drug combination strategies in the context of immune, inflammatory diseases, and cancer.
Gradients of chemoattractive and putative chemorepulsive cues guide cell trafficking in normal and pathological conditions. Lena Biosciences products enable testing of both chemoattractants and chemorepellents in a simple to use 3D cell migration system. In this 3D system, cells can take multiple paths to the same target just as they would in vivo. Next, they can be exposed to multifactorial co- and counter gradients in order to more efficiently direct cells away from certain tissue sites, such as sites of chronic inflammation.
Gradients of chemoattractants and putative chemorepellents guide cell trafficking between vasculature and tissues in normal processes such as leukocyte migration, or extravasation into sites of tissue damage or infection; and in pathological conditions such as abnormal accumulation of inflammatory cells at inflammation sites, leukocyte intravasation from host tissue into vasculature/lymphatics, cancer invasion and metastasis, and transport of intracellular pathogens from infected tissues into the vasculature.
Lena Biosciences SeedEZ and GradientEZ systems enable researchers to model gradient-dependent pathological conditions in 3D including cancer, inflammatory and autoimmune diseases, and to test mono- and drug combination strategies in these models in vitro.
SeedEZ and GradientEZ - simple to use research tools for cell migration assays and advanced 3D cell culture tissue models with immune and inflammatory components.
How? Simply, spot different cell populations, hydrogels, and putative modulators of cell motility, invasion or angiogenesis. You may apply, test and screen multiple test agents and their gradients in parallel, or sequentially with the in situ 3D distributed suspension of cells taxing in 3D, 3D cell cultures, or 3D distributed population of invasive cells invading in 3D. Alternatively, stack the substrates to model multilayered tissues, transendothelial migration, and/or generate gradients. To learn more visit [ Ссылка ]
