Cell Culture can be daunting at first but this video aims to provide some tips and tricks to aid you in successful, contamination-free, cell culture!

This tutorial has a brief overlook of the cell culture process from thawing cells and media changes using aseptic techniques. This video focuses on Jurkat T-Cell suspension cell cultures but the skills and techniques are transferable to adherent cell cultures too. The main difference between adherent and suspension cell cultures is the use of trypsin, an enzyme that digests proteins cells use to adhere to the flask. As the name suggests, adherent cell cultures adhere to the surface so media changes do not need resuspension of cells.

Read more here: [ Ссылка ]

Love this content - Like, Subscribe and let me know in the comments! Notice a problem or mistake? (I am human) Please let me know politely in the comments below! Thanks for watching.

Subscribe: [ Ссылка ]

The content here was filmed at the University of Kent as part of the Optimising Me Manufacturing Systems (OMMS) - EPSRC funded EP/R022534/1. Read more about one of my roles in OMMS here: [ Ссылка ]

Music is HTML by Riot provided by the YouTube Audio Library Free Music.

---------------Contents of this video---------------
00:00 - Intro
00:35 - Preparing the media
02:34 - Cell culture hood Sterilisation (Spray!)
02:51 - Clutter-free cell culture hood
03:29 - Never ever (open media outside the hood)
05:01 - Freezing & Thawing Cells
05:25 - The most important process (Label everything)
06:15 - Aseptic techniques
06:53 - Creating the cell pellet
09:10 - Working inside a cell culture hood
10:33 - Breaking up the cell pellet
11:30 - Removing the supernatant
12:15 - Using a strippette
12:47 - Tidying up the cell culture hood


#Cell #Biology #Culture

свернуть