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In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers - a 2 minute Preview of the Experimental Protocol
Chandirasegaran Massilamany, Arunakumar Gangaplara, Ting Jia, Christian Elowsky, Qingsheng Li, You Zhou, Jay Reddy
University of Nebraska, Lincoln, School of Veterinary Medicine and Biomedical Sciences; University of Nebraska, Lincoln, Center for Biotechnology; University of Nebraska, Lincoln, Nebraska Center for Virology and School of Biological Sciences;
The protocol to detect self-reactive CD4 T cells in brain and heart by direct staining with major histocompatibility complex class II dextramers has been described in this report. For comprehensive analysis, a reliable method to enumerate the frequencies of antigen-specific CD4+ T cells in situ is also devised.
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