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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation - a 2 minute Preview of the Experimental Protocol
John D. Blair, Helen S. Bateup, Dirk F. Hockemeyer
University of California, Berkeley, Department of Molecular and Cell Biology;
Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.
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