Watch the Full Video at [ Ссылка ].
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation - a 2 minute Preview of the Experimental Protocol
John D. Blair, Helen S. Bateup, Dirk F. Hockemeyer
University of California, Berkeley, Department of Molecular and Cell Biology;
Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.
Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: [ Ссылка ]
