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In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition - a 2 minute Preview of the Experimental Protocol

Wei-Ting Ho, Chien-Chia Su, Jung-Shen Chang, Shu-Wen Chang, Fung-Rong Hu, Tzuu-Shuh Jou, I-Jong Wang
Far Eastern Memorial Hospital, Department of Ophthalmology; National Taiwan University, Institute of Clinical Medicine; National Taiwan University Hospital, Department of Ophthalmology; National Taiwan University, College of Medicine;

A primary culture of bovine corneal endothelial cells was used to investigate the mechanism of corneal endothelial-mesenchymal transition. Furthermore, a rat corneal endothelium cryoinjury model was used to demonstrate corneal endothelial-mesenchymal transition in vivo.

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