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Cell Death Associated with Abnormal Mitosis Observed by Confocal Imaging in Live Cancer Cells - a 2 minute Preview of the Experimental Protocol

Asher Castiel, Leonid Visochek, Leonid Mittelman, Yael Zilberstein, Francoise Dantzer, Shai Izraeli, Malka Cohen-Armon
Sheba Medical Center, Cancer Research Center; Tel-Aviv University, The Neufeld Cardiac Research Institute; Tel-Aviv University, Department of Physiology and Pharmacology; Tel-Aviv University, Imaging Unit, Sackler Faculty of Medicine; Ecole Superieure de Biotechnologie Strasbourg, Biotechnology and Cell Signaling; Tel-Aviv University, Department of Human Molecular Genetics and Biochemistry;

The cytotoxic activity of the phenanthridine PJ-34 in cancer cells undergoing mitosis was documented in real time by live confocal imaging. PJ-34 eradicated human breast cancer MDA-MB-231 cells harboring extra-centrosomes in mitosis. Unlike normal bi-focal mitosis, the extra-centrosomes were not clustered in the two spindle poles in the presence of PJ-34.

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