Watch the Full Video at [ Ссылка ].

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells - a 2 minute Preview of the Experimental Protocol

Matteo Fossati, Nica Borgese, Sara Francesca Colombo, Maura Francolini
Fondazione Filarete,; University of Milan, Department of Biotechnology and Translational Medicine; National Research Council (CNR), Neuroscience Institute; "Magna Graecia" University of Catanzaro, Department of Health Science;

We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular compartment.

Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel: [ Ссылка ]

свернуть