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In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans - a 2 minute Preview of the Experimental Protocol
Jessica A. Espino, Lisa M. Jones
University of Maryland, Department of Pharmaceutical Sciences;
In vivo fast photochemical oxidation of proteins (IV-FPOP) is a hydroxyl radical protein footprinting technique that allows for mapping of protein structure in their native environment. This protocol describes the assembly and set-up of the IV-FPOP microfluidic flow system.
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