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High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification - a 2 minute Preview of the Experimental Protocol

Allison Jandura, Jack Hu, Ronit Wilk, Henry M. Krause
University of Toronto, The Terrence Donnelly Centre for Cellular and Biomolecular Research; University of Toronto, Department of Molecular Genetics;

The described RNA in situ hybridization protocol allows the detection of RNA in whole Drosophila embryos or dissected tissues. Using 96-well microtiter plates and tyramide signal amplification, transcripts can be detected at high resolution, sensitivity, and throughput, and at a relatively low cost.

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