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Presynaptically Silent Synapses Studied with Light Microscopy - a 2 minute Preview of the Experimental Protocol
Krista L. Moulder, Xiaoping Jiang, Amanda A. Taylor, Ann M. Benz, Steven Mennerick
Washington University School of Medicine, Department of Psychiatry; Washington University School of Medicine, Department of Anatomy; Washington University School of Medicine, Department of Neurobiology;
Glutamatergic synapses can switch from an active mode to a silent mode. We demonstrate that presynaptic activity status in dissociated culture of rodent neurons is visualized using a fixable form of the FM1-43 dye to visualize active synapses and immunostaining with vGluT-1 antibody to visualize all glutamate synapses.
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