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Live Cell Imaging during Mechanical Stretch - a 2 minute Preview of the Experimental Protocol
Gabriel Rápalo, Josh D. Herwig, Robert Hewitt, Kristina R. Wilhelm, Christopher M. Waters, Esra Roan
University of Tennessee Health Science Center, Department of Physiology; University of Tennessee Health Science Center, Department of Biomedical Engineering and Imaging; University of Memphis, Department of Biomedical Engineering; University of Memphis, Department of Engineering Technology;
A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.
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