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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method - a 2 minute Preview of the Experimental Protocol
Barbora Piknova, Ji Won Park, Katelyn S. Cassel, Cameron N. Gilliard, Alan N. Schechter
NIDDK, NIH, Molecular Medicine Branch;
Nitric oxide (NO) is an important signaling molecule in vascular homeostasis. NO production in vivo is too low for direct measurement. Chemiluminescence provides useful insight into NO cycle via measuring its precursors and oxidation products, nitrite and nitrate. Nitrite / nitrate determination in body tissues and fluids is explained.
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