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A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites - a 2 minute Preview of the Experimental Protocol
Li Xie, Liqin Chen, Pan Gu, Lanlan Wei, Xuejun Kang
Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, School of Public Health of Southeast University; Southeast University, Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering; Tianjin Medical University, School of Public Health; Nanjing Foreign Language School, British Columbia Academy;
We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.
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