Fluorescence light sheet microscopy is a powerful tool to image developmental processes in vivo, but it is limited by the inability to image structures beyond the labelled tissue. In their paper in Development, an Italian-German team (Andrea Bassi from Milan, Benjamin Schmid and Jan Huisken from Dresden) overcome this limitation by combining optical tomography with selective plane illumination microscopy (SPIM). The applications of this approach to the study of organogenesis are obvious in this video. It shows the development of the zebrafish (with fluorescently labelled vasculature) imaged at single-cell resolution with both modalities, from an early embryo (16 hours old) to the larval stage (2 days old). The images were acquired every 10 min and can be seen here from two opposite views. Scale bar is 100 microns.
You can read the paper here: [ Ссылка ]
