Formalin-fixed, 300 micron-thick mouse kidney section was blocked, permeabilized and stained overnight with the following:
Red: Alexa Fluor® 647 anti-mouse CD31 (clone MEC13.3) - [ Ссылка ]
Green: Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3) (clone AA10) - [ Ссылка ]
Blue: Alexa Fluor® 594 anti-mouse Podoplanin (clone Pmab-1) - [ Ссылка ]
All antibodies were used at 5 µg/mL, except for anti-Tubulin Beta 3 (TUBB3) at 2.5 µg/mL. Tissues were optically cleared, then analyzed at 230 µm imaging depth on a confocal microscope.
