0:00 Dengue Virus Virology
--Vector Borne disease, Mosquito Ades aegypti, White bands on legs, Tiger mosquito, Artificial water collection Indoors like aquarium bath tub etc, Bite during day
--RNA Virus, ArboVirus, FlaviViridae single stranded RNA Hemorrhagic Group
1:10 Dengue Pathogenesis
--FEVER THEN RASH Continuous or Saddle back fever, Pain = Break-bone fever, Maculopapular rash
--Neutralizing and Non Neutralizing Antibodies
--Antibody Dependent Enhancement ADE
--Non-structural protein NS1 antigen
2:44 Dengue Old Classification Dengue Fever, DHF Dengue Hemorrhagic fever, DSS Dengue Shock Syndrome
As the extent of capillary leak increases, DSS develops, with a raised haematocrit, tachycardia and hypotension, pleural effusions and ascites.
This may progress to metabolic acidosis and multi-organ failure, including acute respiratory distress
syndrome (ARDS). Minor (petechiae, ecchymoses, epistaxis) or major (gastrointestinal or vaginal) haemorrhage, a feature of DHF, may occur. Cerebrovascular bleeding may be a complication of severe dengue.
3:32 Dengue New Classification - Without warning signs, With Warning Signs, Severe Dengue (Shock)
4:45 Prevention Mosquito Control, Environment, Tetravalent Dengue Vaccine
5:25 Lab Diagnosis,
--NS1 Antigen Detection Non-structural protein
ELISA
ICT = Immunochromatographic test (ICT)
--7:30 Antibody Detection
MAC ELISA = IgM Antibody Capture Enzyme Linked Immunosorbent Assay
--10:12 Virus Detection (correction) RT PCR is Reverse Transcriptase Polymerase chain reaction
Liver Enzymes elevated - alanine aminotransferase (ALT) or aspartate aminotransferase (AST)
HEMATOCRIT/PCV is more.. RBCs proportionately more due to plasma leak
11:30 Summary of Lab Diagnosis
12:47 TREATMENT - fluid replacement, Antivirals not helpful, Dont give aspirin
13:30 Hemorrhagic Fever Cause
14:03 REVISION

#dengue #lab #diagnosis

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