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The movie shows maximum-intensity projections of a DSLM time-lapse multi-view recoding of zebrafish embryonic development, with a view on both the animal and vegetal hemispheres. The wild-type zebrafish embryo was injected with H2B-eGFP mRNA at the one cell stage. Imaging was started in the 64-cell stage. The entire data set consists of 1,226 time points, recorded in 90 s intervals. At each time point, 370 images with 2048 x 2048 pixels each were recorded with a z-spacing of 2.96 mkm. The entire data set comprises 453,620 images (3.5 TBytes). The images were deconvolved with the Lucy-Richardson-algorithm (10 iterations) and normalized for constant overall intensity, thus compensating for the increasing intensity over time due to GFP expression. Detection lens: Carl Zeiss C-Apochromat 10x/0.45 W.
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