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Column chromatography applies the same principles as TLC on a larger scale. In column chromatography, a glass column is filled with a stationary phase (typically silica gel), and the mixture of compounds to be separated is placed on top. Then, a mobile phase (solvent) is passed through the column from top to bottom. As with TLC, the rate at which each compound in the mixture moves down the column depends on its relative attractions to the stationary and mobile phases. View more lessons or practice this subject at [ Ссылка ]
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