#TissueCulture #BananaPropagation #InvitroBanana

In this tutorial I'm going to show you how I perform tissue culture in banana. Im using a protocol by Dr. Frederic Bakry with slightly modifications.

Enjoy!

Tissue cultures were initiated from shoot tips taken from banana suckers. For sterilization, banana suckers were reduced to explants of ˜10 cm in length and 2-4 cm in diameter; they were then immersed in bleach 1% and then 95% (v/v) ethanol, followed by flaming. Flaming
was repeated two times.

Burned tissues are removed and then discarded, in sterile conditions,
up to innermost intact tissues. Shoot apices are slice in two and place in a solid proliferating medium containing:
MS macro- and microelements, vitamins from Morel, 88 mM sucrose, 22.2 µM 6-benzylaminopurine (BAP), 651.5 µM adenine sulphate
and 7 g·L–1 agar.

The pH of the medium is adjusted to 5.8 before autoclaving. Cultures
are kept at 25 - 27 °C in darkness for 45 days.

Active multiplication occurred following
subdivision of bud clusters and subculture
of individual pieces on proliferating medium or rooting medium

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